METHOD
- Dehydrate samples for mounting in DPX by passing them through graded alcohols.
- Add a small drop of DPX to the specimen.
- Remove any excess mount with a paper towel.
- DPX will set almost immediately and the samples can then be observed or photographed.
Is DPX Mountant soluble in Histoclear?
Hystomount, DPX, DePeX and Styrolite were found to be suitable. It was not possible to recommend any one of these in preference to the others. Histoclear is a non-toxic solvent which dissolves most xylene-based mountants as effectively as xylene itself. The use of this product is recommended.
What is DPX cytology?
DPX is a synthetic non-aqueous mounting medium for microscopy. A traditional resin-based slide mountant with xylene solvent. Of medium viscosity and is touch dry in approximately 15 minutes.
Why DPX is used in mounting?
Application. DPX Mountant for histology is a mixture of distyrene, a plasticizer, and xylene used as a synthetic resin mounting media, that replaces xylene-balsam. DPX Mountant dries quickly and preserves stain. DPX Mountant is suitable for HE- (Hematoxylin-Eosin) and Masson-Goldner staining.
How install mount medium?
If you use a long coverslip, you can use a line of mounting medium instead of a drop. Put your slide upside-down and bring it down on the coverslip until it touches the drop of mounting medium and attaches to the coverslip. The medium will spread gradually to the edges of the coverslip without bubbles.
What is DPX in mounting?
DPX Mountant for histology is a mixture of distyrene, a plasticizer, and xylene used as a synthetic resin mounting media, that replaces xylene-balsam. DPX Mountant dries quickly and preserves stain.
What is the meaning of DPX?
Digital Moving Picture Exchange
DPX
| Acronym | Definition |
|---|---|
| DPX | Digital Moving Picture Exchange |
| DPX | Distyrene Plasticizer Xylene (histology) |
| DPX | Digital Picture Exchange |
| DPX | Di-N-Butyle Phthalate in Xylene |
How can I make DPX dry faster?
For faster drying you can leave half an hour at the oven at 60ºC, but be sure it that temperature doesn’t affect the stain quality. DPX is equally good, but not that fast. Usually i leave the slide in the oven at 37ºC overnight just to be sure the cover slip won’t move if I need to use immersion oil.
What is DPX mountant for histology?
DPX Mountant for histology is a mixture of distyrene, a plasticizer, and xylene used as a synthetic resin mounting media, that replaces xylene-balsam. DPX Mountant dries quickly and preserves stain. DPX Mountant is suitable for HE- (Hematoxylin-Eosin) and Masson-Goldner staining.
What is the Thermo Scientific™ DPX mounting media?
The Thermo Scientific™ DPX Mounting Media is used as a synthetic mounting medium in histology and cytology.
How is DPX used in the laboratory?
DPX is dispensed onto a stained specimen slide in liquid form. The evaporation of the carrier solvent results in the hardening of the DPX to form a solid clear film. As DPX has a reflective index similar to glass, the specimen can be viewed under a microscope.
How do I remove the coverslip and DPX from my slides?
The coverslip and DPX can be removed by soaking the mounted slide in a corresponding clearing agent until the coverslip and mounting medium can be removed. Allow the mounted slides to dry for 48 hours prior to filing. This ensures that slides are thoroughly dry and prevents slides sticking together.
