The multiplicity of infection (abbreviated MOI) is the average number of phage per bacterium. The MOI is determined by simply dividing the number of phage added (ml added x PFU/ml) by the number of bacteria added (ml added x cells/ml).
What does an MOI of 1 mean?
The multiplicity of infection or MOI represents the ratio of the numbers of virus particles to the numbers of the host cells in a given infection medium. A value of MOI = 1 implies that on an average there is a single host cell for a single phage particle.
What is MOI and how do you calculate it?
MOI stands for Multiplicity of Infection which refers to the number of viral particles per cell. To calculate, take the number of viral particles used per well then divide by the number of cells originally seeded in the well. This equals the MOI.
What is a titer ratio?
A titer is a measure of how much a sample can be diluted before antibodies can no longer be detected. Titers are usually expressed as ratios, such as 1:256, meaning that one part serum to 256 parts saline solution (dilutant) results in no antibodies remaining detectable in the sample.
What is the titer value?
Serum titers are blood tests that measure whether or not you are immune to a given disease(s). More specifically a quantitative serum titer is a titer with a numerical value indicating your actual degree of immunity to a disease(s).
How do you calculate MOI from IFU?
The MOI is expressed as a ratio of the number of IFU or TU added to the cells (IFU/cell or TU/cell). For example, if your cell line requires an MOI of 200, this would require 200 IFU per 1 cell. LP/mL is a measure of physical titer and will need to be converted to IFU/mL to determine the MOI.
What MOI should I use?
For most cell types, a range of 0.1 – 10 MOI is suitable. For hard to transfect cell lines you may need to increase your range to MOI of 50 or 100.
How do you read titer results?
The greater the concentration of the specific antibody in the serum sample, the higher the titer. For example, a titer for an influenza hemagglutination inhibition assay of 1:10 would be very low; a titer of 1:320 would be high. A low or undetectable titer indicates very little antibody present in the serum.
Which is correct titer or titre?
Titre: Variant spelling of “titer” — the degree of dilution of a substance such as an antibody, reflecting the strength of the solution. Americans spell it “titer” while the English spell it “titre,” French for “title,” derived from the Latin “titulus,” an inscription or label.
What is a normal titer level?
The normal values of an antibody titer depend on the type of antibody. If the testing is done to detect autoantibodies, the normal value should essentially be zero or negative. In the case of testing the efficacy of a vaccine, the normal test result depends on the definite value that is specific for that immunization.
