THP-1 designates a spontaneously immortalized monocyte-like cell line, derived from the peripheral blood of a childhood case of acute monocytic leukemia (M5 subtype) (3). The use of cultured THP-1 cells in vitro as a model for primary human monocytes ex vivo exemplifies the basic concept of translational research.
What are THP-1 cells used for?
THP-1 is a human monocytic cell line derived from an acute monocytic leukemia patient. It is used to test leukemia cell lines in immunocytochemical analysis of protein-protein interactions, and immunohistochemistry.
What is meant by monocyte?
A monocyte is a type of white blood cell and a type of phagocyte. Enlarge. Blood cells. Blood contains many types of cells: white blood cells (monocytes, lymphocytes, neutrophils, eosinophils, basophils, and macrophages), red blood cells (erythrocytes), and platelets.
What does PMA do to monocytes?
When exposed to the phorbol ester PMA, THP-1 monocytes differentiate to mature macrophages. During the differentiation process, NF-κB accumulates in the cytoplasm. LPS stimulation induces the translocation of NF-κB into the nucleus, followed by the secretion of TNF-α.
What are MOLM 13 cells?
MOLM-13 and MOLM-14 are the first cell lines with, and represent the third reported case of, MLL gene rearrangement arising via chromosomal insertion.
What are RAW cells?
RAW 264.7 cells are a macrophage-like, Abelson leukemia virus-transformed cell line derived from BALB/c mice. This cell line is a commonly used model of mouse macrophages for the study of cellular responses to microbes and their products. RAW-Blue™ cells express the secreted alkaline phosphatase (SEAP) reporter gene.
What is THP-1 monocytes?
THP-1 designates a spontaneously immortalized monocyte-like cell line, derived from the peripheral blood of a childhood case of acute monocytic leukemia (M5 subtype) (3). THP-1 cells, including their genetically engineered derivatives, represent valuable tools for investigating monocyte structure and function in both health and disease.
Can I use alternative products for transfection of THP-1 cells?
If you use alternative products, the quality and effectiveness of the item may be affected. These cells are suitable as a transfection host. Tsuchiya S, et al. Establishment and characterization of a human acute monocytic leukemia cell line (THP-1).
How do you harvest undifferentiated THP-1 cells?
Undifferentiated THP-1 cells were harvested by centrifugation for 5 min and 130 × g at 4°C and washed twice with phosphate-buffered saline (PBS).
Does CD14 increase LPs response in THP-1 cells?
Stably transfecting THP-1 cells with human CD14 (10,11) and selecting the transfectants for high-level expression of CD14 results in more pronounced LPS responses of the CD14-transfected THP-1 cells (9).
