APC is well suited for immunohistochemical and immunofluorescence studies of oligodendrocytes and optic nerves due to the antibody’s staining of the cell body as opposed to the myelinated processes.
What is APC antibody?
APC Antibody (F-3) is a high quality monoclonal APC antibody (also designated Adenomatous polyposis coli protein antibody) suitable for the detection of the APC protein of mouse, rat and human origin. APC has been found to be associated with structural components of intracellular junctions.
Are there oligodendrocytes in the corpus callosum?
Oligodendrocyte networks in the corpus callosum provide energy substrates to axons predominantly by delivery of glucose, indicating different metabolic support mechanisms among white matter regions. In the optic nerve, oligodendrocytes maintain axonal function by supplying lactate as an energy substrate.
How many oligodendrocytes are in the brain?
17.4 million oligodendrocytes
Our first finding was that the adult C57BL/6 mouse brain, as a whole, contains around 17.4 million oligodendrocytes (∼20%), against 50.5 million neurons (∼50%) and 27.4 million other cells (∼30%).
Does APC and PE overlap?
In my experience, it is not advisable to use APC/Cy7 and PE/Cy7 together due to heavy cross-beam contamination as their emission max are exactly same. However, they can be used together if you are using machines like the Cytek Aurora.
Is APC brighter than PE?
Compared to PE, APC is not as”bright”. Compared to other red fluorophores such as Cy5, however, APC is still significantly brighter and an excellent choice for use in flow cytometry.
Is APC red?
Allophycocyanin (APC) is an intensely bright phycobiliprotein isolated from red algae that exhibits far-red fluorescence with high quantum yields. It is excited by laser lines at 594 and 633 nm, with an absorbance maximum at 650 nm and a fluorescence emission peak at 660 nm.
What laser is APC?
APC is excited by the red diode laser and excites in several tandem dyes including APC-Cy5. 5 and APC-Cy7. Alexa Fluor 647 (Ex-Max 650 nm/Em-Max 668 nm): conjugates are highly photostable and remain fluorescent over a broad pH range.
Are there oligodendrocytes in the hippocampus?
Oligodendrocytes are unevenly distributed over the hippocampus. White matter areas like the corpus callosum, alveus, and fimbria display strong immunoreactivity. In grey matter areas immunoreactive cells are visible which show different densities in different parts of the hippocampal formation.
What is the difference between oligodendrocytes and Schwann cells?
Oligodendrocytes are the cells which create myelin sheath around the axons of central nervous system. Schwann Cells are the cells which create myelin sheath around the axons of peripheral nervous system. Oligodendrocytes’ main function is the insulation of the nerve axons in the central nervous system.
What are oligodendrocytes cells?
Oligodendrocytes are a type of large glial cell found in the central nervous system. Oligodendrocytes produce the myelin sheath insulating neuronal axons (analogous to Schwann cells in the peripheral nervous system), although some oligodendrocytes (called satellite oligodendrocytes) are not involved in myelination.
Does the monoclonal antibody CC1 bind to APC?
The monoclonal antibody anti-adenomatous polyposis coli (APC) clone CC1, hereafter referred to as the CC1 antibody, is the most common tool used to specifically detect mature oligodendrocytes without labeling myelin; however, previous studies have shown that the CC1 antibody does not bind APC (Brakeman et al. 1999; Lang et al. 2013 ).
Which monoclonal antibody is best for oligodendrocytes?
The mouse monoclonal antibody marketed as anti‐adenomatous polyposis coli clone CC1, often referred to as CC1, is the antibody most commonly used to specifically label mature oligodendrocytes without labeling myelin.
What is anti-APC (AB-7)?
Anti-APC (Ab-7) Mouse mAb (CC-1) Anti-APC (Ab-7), mouse monoclonal, clone CC-1, recognizes APC in oligodendrocytes and astrocytes. It is validated for ICC, IF, free-floating Sections, and paraffin and frozen sections. – Find MSDS or SDS, a COA, data sheets and more information.
Does the CC1 antibody bind to qki7?
Further supporting the conclusion that the CC1 antibody binds QKI7, CC1 staining is absent from mouse brains following genetic disruption of the qkI gene (Darbelli et al. 2016). QKI7 also labeled a small number of cells not labeled by the CC1 antibody, including Bergmann glia in the Purkinje cell layer (Fig.
