The Wade-Fite staining technique is a less common special staining useful in Histopathology. It is the modification of Ziehl-Neelsen Staining Method to demonstrate Mycobacterium leprae in tissue sections, which is much less acid and alcohol fast than the tubercle bacilli.
What is the principle of Zn stain?
PURPOSE: Used in the demonstration of acid-fast bacteria belonging to the genus ‘mycobacterium’, which include the causative agent for tuberculosis. PRINCIPLE: The lipoid capsule of the acid-fast organism takes up carbol- fuchsin and resists decolorization with a dilute acid rinse.
How do you perform a Zn stain?
Ziehl Neelsen Acid-fast stain
- Step 2: Smear Preparation (Review)
- Cover the smear with carbolfuchsin dye.
- Dry heat for 2 minutes.
- Cool and rinse with water.
- Wash the top and bottom of slide with water and clean the slide bottom well.
- Counterstain with Methylene Blue for 30 seconds to 1 minute.
What is Fite Faraco?
The Fite Faraco technique is the oldest method used to detect Mycobacterium leprae in tissue specimens. Mycobacterium tuberculosis is diagnosed using the Ziehl-Neelsen Stain/Acid-Fast Stain which differentiates acid-fast from non-acid-fast bacilli.
What is AFB blood test?
AFB smear—a microscopic examination of a person’s sputum or other specimen that is stained to detect acid-fast bacteria. It is a rapid test used to provide presumptive results within one to two days. It is valuable in helping to make decisions about treatment while waiting for culture results.
What is Zn technique?
The Ziehl Neelsen (ZN) method of staining acid fast bacilli has been in vogue for more than hundred years. In the ZN method [1], the basic fuchsin phenol dye is used hot there by melting the unsaponifiable waxy substance on the surface of the cell wall.
Why do we heat in ZN staining?
When the smear is stained with carbol fuchsin, it solubilizes the lipoidal material present in the Mycobacterial cell wall but by the application of heat, carbol fuchsin further penetrates through lipoidal wall and enters into cytoplasm.
What are the procedural steps of Ziehl neelsen?
Ziehl-Neelsen (Acid Fast) Staining procedure
- Spread the sputum evenly over the central area of the slide using a continuous rotational movement.
- Place slides on the dryer with smeared surface upwards, and air dry for about 30 minutes.
- Heat fix dried smear.
- Cover the smear will carbol fuchsin stain.
What are the uses of Fite?
PURPOSE: To demonstrate mycobacterium leprae (leprosy), which are acid fast organisms. PRINCIPLE: This technique combines peanut oil with the deparaffinizing solvent (xylene), minimizing the exposure of the bacteria’s cell wall to organic solvents, thus protecting the precarious acid-fastness of the organism.
Which test is used for leprosy?
A lepromin skin test is used to determine the type of leprosy a person has contracted. The lepromin skin test is also called the leprosy skin test. Leprosy is a long-term (chronic) condition caused by the bacteria Mycobacterium leprae.
