H358 is a non-small cell lung cancer (NSCLC) cell line that harbors a KRAS mutation. KRAS mutations are found in >25% of lung adenocarcinomas leading to constitutive activation of the growth-promoting RAS signaling pathway. H358 cells were established from a chemotherapy-naïve NSCLC tumor.
What is NCI H460?
The NCI-H460 cell line was derived by A.F. Gazdar and associates in 1982 from the pleural fluid of a patient with large cell cancer of the lung. Gender Male Karyotype. modal numbr = 57; range = 53 to 65. This is a hypotriploid human cell line.
Is A549 cell line metastatic?
Among NSCLC cells, A549 formed the least amount of metastatic lesions (0.9 ± 0.35 cells per HPF), while H1299, a metastatic cell line, formed the maximum number of metastatic lesions (3.6 ± 1.03 cells per HPF).
Why do we use A549 cells?
The human A549 adenocarcinoma cell line has been used in lung cell biology as a model for ATII cells. These highly specialized cells produce surfactant, a multifunctional lubricant that reduces surface tension and prevents alveolar collapse during ventilation.
Where is carcinoma found?
Carcinoma is the most common type of cancer. It begins in the epithelial tissue of the skin, or in the tissue that lines internal organs, such as the liver or kidneys. Carcinomas may spread to other parts of the body, or be confined to the primary location.
How do I freeze A549 cells?
Distribute 1 ml of the cell suspension in freezing medium into each cryovial. Place cryovials in a freezing container and place this into a freezer (-70°C to -80°C) for 24 h. This leads to a freezing rate of approximately 1°C/min. After 24 h place the frozen cryovials into liquid nitrogen for long term storage.
How long does it take A549 cells to adhere?
24-40 hours
A549 Cell Subculture Protocol A549 cells are cultured in complete media consisting of Dulbecco’s MEM modified with 10% FBS. The cells can be grown as adherent or in suspension in vitro. The A549 cell line grows easily and cell count doubling time is typically 24-40 hours.
What does NCI h358 stand for?
High molecular weight genomic DNA isolated from the NCI-H358; human bronchioalveolar carcinoma; non-small cell lung cancer cell line. Discounts are available for our fellow nonprofit organizations.
Does ncnci-h358 Express UDP-glucuronosyltransferases?
NCI-H358 do not express UDP-glucuronosyltransferases, but do express glutathione-S-transferase and phenol sulphotransferase. Expression of SP-A protein and RNA, the major surfactant-associated protein was detected. SP-B and SP-C RNA was not expressed.
Is the information on the ATCC product sheet accurate?
Any proposed commercial use is prohibited without a license from ATCC. While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only.
