RedSafe™ Nucleic Acid Staining Solution (20,000x) is a new and safe nucleic acid staining solution. It is an alternative to the traditional ethidium bromide(EtBr) stain for detecting nucleic acid in agarose gels. It emits green fluorescence when bound to DNA or RNA.
How much RedSafe should I add?
Add 5 ㎕ of RedSafeTM Nucleic Acid Staining Solution (20,000x) to the agarose solution. Swirl the flask gently to mix the solution and avoid forming bubbles. 3.
What is the purpose of the use of the Red Safe DNA stain?
Safe-Red™ is a new and safe nucleic acid stain for the visualization of nucleic acids in agarose and polyacrylamide gels. This dye eliminates the need for toxic Ethidium Bromide (EtBr, a potent mutagen), commonly used in gel electrophoresis.
How does SYBR Safe stain work?
SYBR Safe is a cyanine dye used as a nucleic acid stain in molecular biology. SYBR Safe binds to DNA. The resulting DNA-dye-complex absorbs blue light (λmax = 509 nm) and emits green light (λmax = 524 nm).
Is RedSafe carcinogenic?
Thus RedSafe can be considered non-carcinogenic. Most importantly, RedSafe is not considered hazardous waste, and can be disposed of according to standard laboratory procedures.
What is novel juice?
Novel Juice is a non-mutagenic fluorescent reagent that produces instant visualization of DNA bands upon Blue Light or UV illumination of agarose gels. Supplied in GeneDireX’s 6X DNA Loading Buffer, Novel Juice is used to prepare DNA markers and samples for loading on agarose or polyacrylamide gels.
How much GelRed do you add?
A 0.5 mL vial of GelRed® or GelGreen® is can be used to prepare 100 minigels (50 mL each) using the precast protocol, or for post-electrophoresis staining of 33 minigels in 50 mL staining solution per gel. Post-staining solution also can be re-used for staining two or more gels.
How do you use red safely?
AThank you for your interest in our products. This product is a new and safe nucleic acid staining reagent as a substitute for EtBr (ethidium bromide) used to identify nucleic acid in Argarose gel, and it emits green fluorescence. Samples will be processed through our sales representative as soon as possible.
What is the purpose of DNA stain DNA red in gel electrophoresis?
Preparing the DNA for electrophoresis A dye is added to the sample of DNA prior to electrophoresis to increase the viscosity of the sample which will prevent it from floating out of the wells and so that the migration of the sample through the gel can be seen.
Is SYBR Safe really safe?
SYBR® Safe It is marketed as being less harmful than ethidium bromide, but this is debatable. Its major advantage is that it is as sensitive as ethidium bromide but does not require UV light for visualization.
How much DNA can SYBR Safe detect?
What is the lower limit of detection of SYBR Safe DNA Gel Stain? SYBR Safe DNA Gel Stain yields the same sensitivity as ethidium bromide—roughly 500 pg/band in a minigel, for fragments larger than 200 bp viewed on a 300 nm transilluminator.
Are gel stains safe?
A number of dyes have been marketed as being safe because they are not mutagenic in lab tests. But most of these so-called safe gel stains contain well-known, old-fashioned fluorescent dyes, many of which are highly membrane permeable, so they penetrate and bind DNA inside of live cells within minutes.
