Spherisorb analytical columns are durable, high-efficiency chromatographic columns, featured in thousands of references in chromatographic literature. The wide range of column lengths and diameters offers you exceptional flexibility in optimizing methods and reducing solvent consumption.
What is ODS1?
Spherisorb ODS1 columns are general purpose, silica-based, reversed-phase C18 columns. The ODS1 packing features low ligand density with a specified carbon load of 6.2%.
Is analytical column used in HPLC?
An HPLC typically includes two columns: an analytical column responsible for the separation and a guard column. The guard column is placed before the analytical column, protecting it from contamination.
What is ODS2 column?
Spherisorb ODS2 columns are general purpose, silica-based, reversed-phase C18 columns. The ODS2 packing features intermediate ligand density with a specified carbon load of 11.5%.
What is an analytical column?
“Analytical columns” are meant for qualitative analyses. The chromatograms obtained from the use of analytical columns gives qualitative profile or fingerprinting of the sample. Prior to carrying out ‘preparative chromatography’ runs, it is good to get a profile/fingerprint of the sample by analytical chromatography.
Why silica is used in HPLC column?
Generally, silica gel is filled in the high-performance liquid chromatography columns because of its particle size and porosity that helps in separation of components and silica gel is also an inert material that does not react with mobile phases. The material filled in the HPLC columns is known as a stationary phase.
What is the role of column packing?
Packing offers the advantage of a lower pressure drop across the column (when compared to plates or trays), which is beneficial while operating under vacuum. Differently shaped packing materials have different surface areas and void space between the packing. Both of these factors affect packing performance.
Why is silica used in column chromatography?
Silica and alumina are both polar adsorbents so the more polar components in the mixture to be separated are retained more strongly on the stationary phase and are therefore eluted from the column last. Silica is recommended for most compounds, but as it is slightly acidic, it preferentially retains basic compounds.
What is inertsil column?
Inertsil ODS columns are general purpose, reversed phase C18 columns available in 5 μm and 10 μm particle sizes. It was the first ODS bonded phase introduced from GL Sciences back in 1986. We recommend InertSustain C18 or Inertsil ODS-4 columns for all new method development.
What is the difference between inertsil ODS 3 and ODS 3V?
Inertsil ODS-3V offers all of the outstanding chromatographic benefits of Inertsil ODS-3 with the added benefit of a more thoroughly documented, validated QC procedure consistent with the demands of GLP/GMP compliance.
What is analytical and preparative column?
“Preparative columns” are meant for isolating compounds from natural (product) extracts. It is meant for purifying compounds at a large scale, say in terms of ‘milligram’ or ‘gram’. “Analytical columns” are meant for qualitative analyses. The eluents from the analytical column may not have to be collected.
What is a waters spherisorb column?
Waters Spherisorb Columns are produced in a wide range of particle sizes (3, 5, and 10 µm) and bonded phases to meet your chromatographic needs. In addition, Waters their high-quality bonded phases give many different and unique separation selectivities.
Why choose the spherisorb silica column?
Designed to understand and address the effects of all process parameters such as slurry solvent choice, packing pressure time, temperature, flow rate, and slurry volume, the Spherisorb Silica Column is the ideal addition to your wide range of lab equipment.
What is spherisorb ods1?
Spherisorb ODS1 columns are general purpose, silica-based, reversed-phase C18 columns. The ODS1 packing features low ligand density with a specified carbon load of?6.2%. Spherisorb ODS1 columns are general purpose, silica-based, reversed-phase C18 columns.
What is the difference between spherisorb NH2 and C1?
Spherisorb C1 columns are classified as USP L13 and are general purpose columns that contain trimethylsilane bonded to silica. Spherisorb NH2 (Amino) columns contain a silica-based aminopropyl bonded sorbent.
