SmaI is a restriction endonuclease used to cut DNA at the recognition sequence 5′-CCC/GGG-3′, generating DNA fragments with blunt termini.
Is SmaI a good restriction enzyme?
DNA Restriction Enzymes from Takara such as SmaI are high-quality: perform restriction enzyme digestion with reliable restriction endonucleases.
What is restriction site analysis?
Restriction Site Analysis (RFLPs) A restriction site is a sequence of approximately 6–8 base pairs of DNA that binds to a given restriction enzyme. These restriction enzymes, of which there are many, have been isolated from bacteria. Their natural function is to inactivate invading viruses by cleaving the viral DNA.
What is SmaI restriction enzyme?
SmaI (MB075) Description: SmaI is a Restriction Endonuclease purified from an Escherichia coli strain that carries the SmaI gene from Serratia marcescens. SmaI activity is blocked by CpG methylation. NZYTech’s SmaI is an enzyme that acts at 25 °C.
What is the recognition site for SmaI?
Thermo Scientific SmaI restriction enzyme recognizes CCC^GGG sites and cuts best at 30°C in Tango buffer. See Reaction Conditions for Restriction Enzymes for a table of enzyme activity, conditions for double digestion, and heat inactivation for this and other restriction enzymes.
What is the recognition sequence for SmaI?
The SmaI and XmaI endonucleases are imperfect isoschizomers that recognize the sequence CCCGGG.
Is SmaI blunt end?
You can also simply cut the DNA with a restriction endonuclease that leaves blunt ends. SmaI and EcoRV are two such endonucleases that can be used to linearize circular plasmids or cut off sticky ends leaving blunt ends ready for ligation (Fig.
What do you mean by restriction analysis?
the analysis of DNA molecules by the use of RESTRICTION ENZYMES. (d) to construct physical maps, called restriction maps, of DNA molecules, that indicate the position of restriction sites in the molecules. …
How can you tell if a site is restrictions?
The option Find Restriction Sites… from the “Tools”→“Cloning” menu or the context menu allows you to find and annotate restriction sites on a nucleotide sequence.
What type of ends does SmaI produce?
SmaI is an isoschizomer of XmaI. SmaI produces blunt-ended fragments whereas XmaI produces a 5´ extension.
What does SmaI mean?
SMAI
| Acronym | Definition |
|---|---|
| SMAI | Societe de Mathematiques Appliquees et Industrielles (French) |
| SMAI | Styrene Maleic Anhydride Imide (resin) |
What is restriction analysis used for?
Restriction analysis is to identify restriction mapping sites in DNA sequences using appropriate enzyme sets and enzyme filtering criteria as per specific experimental requirements.
What is SMAI in E coli?
An E. coli strain that carries the SmaI gene from Serratia marcescens (ATCC 49779). One unit is defined as the amount of enzyme required to digest 1 µg of λ DNA (HindIII digest) in 1 hour at 25°C in a total reaction volume of 50 µl. SmaI is an isoschizomer of XmaI.
What is the difference between SmaI and XmaI?
One unit is defined as the amount of enzyme required to digest 1 µg of λ DNA (HindIII digest) in 1 hour at 25°C in a total reaction volume of 50 µl. SmaI is an isoschizomer of XmaI. SmaI produces blunt-ended fragments whereas XmaI produces a 5´ extension. SmaI has a half-life of 15 minutes at 37°C. Blocked by CpG methylation.
What is restriction analyzer?
Restriction Analyzer is an online restriction analysis tool. It scans a DNA sequence for the presence of restriction sites and outputs tabular results and an annotated sequence. It also calculates the lengths of restriction fragments and displays the fragment pattern as a virtual agarose gel.
How do I enter a list of restriction enzymes for analysis?
If a specific set of restriction enzymes is to be used in the analyses, one can either enter the list (paste it in the text area in the “Enter the restriction enzyme list” dialog) or load a list of enzymes provided as a plain text file with one restriction enzyme name per line, for example:BamHIEcoRIHindIIIetc. Close DNA SOURCE GENOTYPE
